Interference by goat-derived anti-lymphocyte globulin in a double-antibody radioimmunoassay of digoxin.
نویسندگان
چکیده
Therapy with anti-lymphocyte and anti-thymocyte globulins, two animal-derived preparations used as immunosuppressive agents, results in high concentrations of foreign immunoglobulin circulating in plasma. We describe a case that illustrates how the presence of these immunoglobulins can interfere with double-antibody radioimmunoassay procedures.
منابع مشابه
Double-AntibodyRadioimmunoassayfor Factor Vill-Related Antigen
A plasma protein required for the support of ristocetininduced platelet aggregation was isolated from antihemophilic factor concentrate and radiolabeled with 1251. A double-antibody radioimmunoassay was developed, with use of specific rabbit anti-Vill related antigen serum and goat anti-rabbit globulin. The assay is sensitive, reproducible, and technically simple to perform. Values obtained in ...
متن کاملInterference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin.
Although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. We investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. Carrier-free 125I-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free frac...
متن کاملRelationship between post-transplant lymphoproliferative disorder and Anti-Thymocyte Globulin or Anti-Lymphocyte Globulin
Abstract Background: Lymphoproliferative disorders are among the most serious and potentially fatal complications of chronic immunosuppression in kidney transplant recipients. The principle risk factors for development of PTLD are the degree of overall immunosuppression and the EBV serostatus of the recipient. In this study, the risk of PTLD in kidney transplant recipients who received Anti- L...
متن کاملImmunological Considerations in the Use of Goat Antibodies to Carcinoembryonic Antigen for the Radioimmunodetection of Cancer1
Preparation of radioantibodies from carcinoembryonic anti gen (CEA) antisera raised in goats by conventional procedures necessitates extensive absorption procedures to remove anti body activity against CEA-related substances. Further purifi cation by affinity chromatography increases the antibody con tent of the radiolabeled preparation and enhances antibody uptake by a xenogeneic tumor. In pat...
متن کاملGlomerular basement membrane--reactive antibody in anti-lymphocyte globulin.
Equine immunoglobulin was detected along the glomerular basement membrane of three human homograft recipients who had been treated with equine anti-lymphocyte globulin. Anti-lymphocyte globulins, given these patients, were obtained by immunization of horses with lymphocytes from human spleens and/or lymph nodes and contained glomerular basement membrane-reactive antibodies. Quantitative paired-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 33 4 شماره
صفحات -
تاریخ انتشار 1987